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Extender based on caseins with addition of cholesterol. Milk casein phosphoproteins protects sperm cells from the action of seminal plasma proteins (HSP-1, HSP-2) and Ca++ ions, prolonging spermatic longevity favoring semen transport.
The temperature of 5 degrss C or 15 degrees C can be used for semen cooled transport as well as for pre-freezing centrifugation. Use dilutions of 50 million/ml for cooled transport, respecting the minimum of 2 parts of extender to 1 part of semen.
In case of samples requiring centrifugation before cooled transport should be respected the resuspension of spermatozoa to 100 million/ml. DO NOT exceed the appropriate concentrations of cholesterol.
Paper reports that seminal samples diluted in casein presents a higher rate of binding to the oocyte. Suitable for all stallions, especially for those with suspected premature sperm capacitation.
BotuSemen Gold presented liguid translucent which facilitates visualization for cushion centrifugation.