Extender based on caseins with addition of cholesterol. Milk casein phosphoproteins protects sperm cells from the action of seminal plasma proteins (HSP-1, HSP-2) and Ca++ ions, prolonging spermatic longevity favoring semen transport.
The temperature of 5 degrss C or 15 degrees C can be used for semen cooled transport as well as for pre-freezing centrifugation. Use dilutions of 50 million/ml for cooled transport, respecting the minimum of 2 parts of extender to 1 part of semen.
In case of samples requiring centrifugation before cooled transport should be respected the resuspension of spermatozoa to 100 million/ml. DO NOT exceed the appropriate concentrations of cholesterol.
Paper reports that seminal samples diluted in casein presents a higher rate of binding to the oocyte. Suitable for all stallions, especially for those with suspected premature sperm capacitation.
BotuSemen Gold presented liguid translucent which facilitates visualization for cushion centrifugation.
100mg gentamicin and 100mg penicillin per 100ml.