Extender based on skimmed milk with addition of Cholesterol. Cholesterol is a componet of the spermatic membrane that contributes for and increas in its stability. It makes Botu-Semen Special highly recommended for pre-freezzing centrifugation.
The temperature of 5 degrees C or 15 degrees C can be used for semen cooled transport as well as for pre-freezing centrifugation. Use dilutions of 30 to 50 million/ml for cooled transport, respecting the minimun of 2 parts of extender to 1 part of semen.
In case of samples requiring centrifugation beofre cooled transport should be respected the resuspension of spermatozoa to 100 milliom/ml. DO NOT exceed the appropriate concentrations of cholesterol.
Suitable for all stallions, especially for spermatozoa that presents sensitivity to cooled transport.
100mg gentamicin and 100mg penicillin per 100ml.